Institut für Phytomedizin

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Browsing Institut für Phytomedizin by Person "Bantleon, Georg"

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    Extensive efficacy tests of non-antibiotic fire blight control agents
    (2020) Bantleon, Georg; Scheer, Christian; Hartung, Karin; Vögele, Ralf
    Application of control agents to prevent new infections is an integral part of disease management for fire blight on pome trees caused by Erwinia amylovora (Burrill) Winslow et al.. Due to resistance development and human health concerns when using antibiotics, non-antibiotic control agents with high efficacy are urgently needed. In this study extensive efficacy tests with some classical antibiotic and relatively new non-antibiotic control agents for the prevention of bloom and shoot blight as well as phytotoxicity tests based on fruit russeting were carried out using young apple trees. Furthermore an experimental design for testing control agents preventing fire blight following hail injury was developed. The high efficacy of streptomycin (> 90%) and streptomycin containing spray strategies (> 80%) was confirmed. Non-antibiotic agents with high efficacy are LMA (85%), a potassium aluminum sulfate, and Juglon (80%). LMA is already in use in practice in Austria and Germany with a temporal and restricted permission and the administrative process to gain the legal status of a pesticide is ongoing whilst already admitted in Switzerland. Juglon, an organic compound from walnut, did show a high efficacy, but no consistent results (80.0 and 69.5%). Myco-Sin showed good results to prevent infection after artificial hail simulation. Antinfek showed high efficacy (90%) in preventing bloom blight, but could be tested only once so further research is needed to confirm this result. The design of the presented study enabled an extensive evaluation of the potential control agents and the aggregation and comparison of results and can be advised for other evaluations.
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    Feuerbrand : Charakterisierung und Bekämpfungsmaßnahmen
    (2012) Bantleon, Georg; Vögele, Ralf
    This work provides new scientific findings on control agents for fire blight after hail and during bloom, on fire blight susceptibility of modern apple cultivars and on fire blight pathogenesis of blossom infection and of internal stem movement. Experiments were conducted in the laboratory, greenhouse and field. Leaf damages after hail are points of entry for new infections. Until now, there has been no method established for testing control agents after hail injury. In the method developed, plants were damaged and sprayed with a suspension of Erwinia amylovora. Control agents were applied 4 h after inoculation. The visual symptoms were scored. Streptomycin showed high effectivity. Of 13 agents tested, 3 showed an effectivity sufficient for practical use (Juglon, LMA und Myco-Sin). Flowers are the main point of entry for Erwinia amylovora. Control agents for fire blight were tested according to EPPO standard PP 1/166(3). The goal was to identify alternatives to the antibiotic streptomycin which won?t be available in fruit-growing in the future. Out of 20 agents tested, streptomycin always showed high effectivity as well as spray strategies involving streptomycin. Three agents were found to be potential substitutes for streptomycin (Antinfek, Juglon und LMA). Eight modern apple cultivars were tested for their fire blight susceptibility in flower tests in the climate chamber and in the field. Rewena showed low, Mairac and Pinova medium, Diwa, Gala Greenstar and Kanzi high and Wellant very high susceptibility. The results of the climate chamber and results from field trials correspond. A clear relation between the density of the inoculation suspension and symptom development in flower and shoot was found in pathogenesis experiments. All concentrations led to infections. Higher concentrations led to more severe symptoms. In another pathogenesis experiment bacteria were injected into the stems of apple trees. Stem tissue was analyzed for Erwinia amylovora using real-time PCR. Bacteria could only be found below the point of injection what favors the idea of phloem migration.