Browsing by Person "Sprau, Lina"

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Honey bees (Apis mellifera) preselected for Varroa sensitive hygiene discriminate between live and dead Varroa destructor and inanimate objects
(2023) Sprau, Lina; Traynor, Kirsten; Rosenkranz, Peter; Sprau, Lina; State Institute of Bee Research, University of Hohenheim, Stuttgart, Germany; Traynor, Kirsten; State Institute of Bee Research, University of Hohenheim, Stuttgart, Germany; Rosenkranz, Peter; State Institute of Bee Research, University of Hohenheim, Stuttgart, Germany
Varroa destructor is one of the main causes of colony losses of the western honey bee ( Apis mellifera ). Many efforts exist to breed honey bees resistant to V. destructor . Varroa sensitive hygiene (VSH) is a commonly selected behavioural trait; VSH workers remove the pupae of mite infested brood cells with high efficiency, interrupting the reproduction of the mite. The cues and triggers for this behaviour are not yet fully understood. To determine what elicits this removal behaviour, we examined preselected VSH workers´ responses to four different groups of objects inserted into freshly capped cells: live mites, dead mites, odour reduced mites, and glass beads. These were also compared to control cells that were opened and closed without inserting any object. The pupae in cells containing inorganic objects (glass beads) were removed at similar rates to the control, demonstrating that an object alone does not trigger a removal response. Dead and odour reduced mites were removed at a higher frequency than control cells, but less frequently than live mites. Workers sometimes removed items resting near the top of the cell without removing the pupa. Our results demonstrate that although mite odour from dead mites triggers removal behaviour, the pupa of cells containing live mites were removed more frequently, suggesting that other cues (i.e. odour from feeding wound) or signals (i.e. pupal movement to signal distress) are important. Future research should focus on elucidating these other cues or signals from the brood and mites, as mite presence alone seems to be insufficient.
Standard Methods for Dissection of Varroa destructor Females
(2021) Piou, Vincent; Vilarem, Caroline; Rein, Carolin; Sprau, Lina; Vétillard, Angélique
Varroa destructor (Anderson and Trueman) is known as a major pest of Apis mellifera L, especially in the Northern Hemisphere where its effects can be deleterious. As an obligate parasite, this mite relies entirely on its host to reproduce and complete its cycle. Studies focusing on isolated organs are needed to better comprehend this organism. To conduct such targeted molecular or physiological studies, the dissection of V. destructor mites is crucial as it allows the extraction of specific organs. Here, we propose a technical article showing detailed steps of females V. destructor dissection, illustrated with pictures and videos. These illustrated guidelines will represent a helpful tool to go further in V. destructor research.