Landessaatzuchtanstalt

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Browsing Landessaatzuchtanstalt by Sustainable Development Goals "3"

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    Comparative quantitative LC–MS/MS analysis of 13 amylase/trypsin inhibitors in ancient and modern Triticum species
    (2020) Geisslitz, Sabrina; Longin, C. Friedrich H.; Koehler, Peter; Scherf, Katharina Anne
    Amylase/trypsin inhibitors (ATIs) are major wheat allergens and they are also implicated in causing non-celiac gluten sensitivity and worsening other inflammatory conditions. With only few studies on ATI contents in different Triticum species available so far, we developed a targeted liquid chromatography-tandem mass spectrometry (LC–MS/MS) method based on stable isotope dilution assays to quantitate the 13 most important ATIs in a well-defined sample set of eight cultivars of common wheat and durum wheat (modern species), as well as spelt, emmer and einkorn (ancient species) grown at three locations in Germany, respectively. Only few ATIs with low contents were detected in einkorn. In contrast, spelt had the highest total ATI contents. Emmer and common wheat had similar total ATI contents, with durum wheat having lower contents than common wheat. Due to the lack of correlation, it was not possible to estimate ATI contents based on crude protein contents. The wheat species had a higher influence on ATI contents than the growing location and the heritability of this trait was high. Despite comparatively low intra-species variability, some cultivars were identified that may be promising candidates for breeding for naturally low ATI contents.
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    Influence of variety and growing location on carotenoid and vitamin E contents of 184 different durum wheat varieties (Triticum turgidum ssp. durum) in Germany
    (2020) Groth, Sabrina; Wittmann, Ramona; Longin, C. Friedrich H.; Böhm, Volker
    The influence of variety and growing location on the carotenoid and vitamin E content of 184 different varieties of durum wheat of each of the German locations Hohenheim and Seligenstadt was analyzed by HPLC. In addition, the yellow pigment content was measured as b value using a chroma meter. The results showed that the measured parameters vary both between sites and varieties, with higher variance between varieties. Finally, we elaborated a high genetic variance and heritability for lutein and total carotenoids and no negative correlations to important agronomic and quality traits in durum wheat. Thus, future durum breeding could produce varieties with improved agronomy, quality, and increased contents of lutein and total carotenoids. Vitamin E has only a minor importance due to the low contents in durum wheat. Due to the high correlation between the b value and the total carotenoid content, the b value could be used as a cheap and rapid method to initially screen high numbers of breeding lines before testing individual promising breeding lines with HPLC, warranting efficient and accurate selection of durum lines with increased carotenoid content.