Browsing by Subject "Vitamin C"

Now showing 1 - 3 of 3

Combined effects of drought and soil fertility on the synthesis of vitamins in green leafy vegetables
(2023) Park, Taewan; Fischer, Sahrah; Lambert, Christine; Hilger, Thomas; Jordan, Irmgard; Cadisch, Georg
Green leafy vegetables, such as Vigna unguiculata, Brassica oleraceae, and Solanum scabrum, are important sources of vitamins A, B1, and C. Although vitamin deficiencies considerably affect human health, not much is known about the effects of changing soil and climate conditions on vegetable vitamin concentrations. The effects of high or low soil fertility and three drought intensities (75%, 50%, and 25% pot capacity) on three plant species were analysed (n = 48 pots) in a greenhouse trial. The fresh yield was reduced in all the vegetables as a result of lower soil fertility during a severe drought. The vitamin concentrations increased with increasing drought stress in some species. Regardless, the total vitamin yields showed a net decrease due to the significant biomass loss. Changes in vitamin concentrations as a result of a degrading environment and increasing climate change events are an important factor to be considered for food composition calculations and nutrient balances, particularly due to the consequences on human health, and should therefore be considered in agricultural trials.
GLUT-1 content and interaction with stomatin in red blood cells from species without vitamin C biosynthesis and their relevance for diabetes mellitus type 1
(2016) Frey, Tabea Caroline; Biesalski, Hans-Konrad
Ascorbic acid is commonly known as vitamin C. By definition, vitamins, with the exception of vitamin D, are substances which can not be synthesized, but are essential for the organism. In this light, vitamin C is special. It is hypothesized that millions of years ago some species, like primates, Guinea pigs and fruit bats, lost the ability to synthesize ascorbate from glucose due to an inactivation of an enzyme called L-gulono-g-lactone oxidase. Since then, these species have been dependent on dietary intake of this micronutrient. Ascorbate is not only the most efficient watersoluble antioxidant, but also an important cofactor in neurotransmitter or collagen biosynthesis. An inadequate intake of this vitamin leads to scurvy. In 2008, a French researcher documented that all species that lost the ability to synthesize ascorbic acid express a different facilitative glucose transporter isoform(GLUT) in their erythrocytes. The expressed GLUT-1 transports not only glucose but also dehydroascorbate which is the oxidized form of vitamin C. Was that different GLUT expression the keystone event for the evolutionary success of these species? To examine the questions regarding the evolutionary benefit of this transporter expression, the kinetics of ascorbate and dehydroascorbate transport into erythrocytes from four different species were evaluated. Further, recycling and disposal of the transported vitamin as well as a possible accumulation were observed. The results demonstrate that there are three different transport types of dehydroascorbate. One which does not transport the vitamin at all, one whose transport of dehydroascorbate competes with glucose and one which absorbs dehydroascorbate completely independent of the extracellular glucose concentration. After absorption, vitamin C is not recycled and is not disposed back into the extracellular fluid. Additionally, it is not stored in the cell until the erythrocyte undergoes apoptosis. The evolutionary benefit is found in an electron transfer across the erythrocyte membrane from intracellular ascorbate to the extracellular, oxidized form of vitamin C. In an energetic light, this recycling of extracellular vitamin C is more efficient than the de novo synthesis of the micronutrient. Therefore, the erythrocyte acts not as a reservoir for vitamin C storage, but as a reservoir for electron storage to prevent degradation and loss of dehydroascorbate in times of high oxidative stress. This electron reservoir becomes more important in diseases with high levels of oxidative stress. A metabolic disorder, which is frequently described to be accompanied by high levels of oxidative stress and lowered vitamin C levels in plasma and cells, is Diabetes mellitus. The decreased plasma concentrations do not result from a smaller dietary intake. Probably, the uptake of dehydroascorbate into erythrocytes, and, therefore, the extracellular ascorbate recycling is disordered. Investigations of the distribution of GLUT-1 in different erythrocyte membrane subdomains showed that the regulation of this transporter is altered in subjects with diabetes mellitus type 1 compared to healthy controls. In vitro, no differences in dehydroascorbate transport rate could be observed, but significantly decreased intra-erythrocyte vitamin C concentrations were detected in vivo. In conclusion, the altered regulation of GLUT-1 in the erythrocyte membrane in the case of diabetes can affect vitamin C recycling in plasma. A decreased ascorbate pool in the cells leads to a decreased recycling capacity, and, therefore, to a lower antioxidant defense outside the cell. Due to that knowledge, the recommended dietary intake of vitamin C in the case of diabetes mellitus must be reconsidered to prevent further complications.
Role of iron and TfR1 in the application of high‑dose ascorbate against pancreatic cancer
(2026) Piotrowsky, Alban; Leischner, Christian; Schmieder, Hendrik; Detert, Katja; Schneider, Kathrin; Schulte, Johanna; Hammerschmidt, Sabrina; Marongiu, Luigi; Renner, Olga; Burkard, Markus; Venturelli, Sascha
Pancreatic cancer remains one of the deadliest tumor diseases with an urgent need for new therapy options. At the same time, the use of high‑dose vitamin C in cancer treatment has been investigated for decades. Despite promising in vitro and in vivo data and initial clinical studies, there is a need for optimization with regard to an ideal treatment regimen and suitable patient population for the use of high‑dose vitamin C. The aim of the present study was to evaluate for the first time the combination of high‑dose vitamin C with the administration of iron in three human pancreatic cancer cell lines and to determine the exact cell death mechanism. While the investigated cell lines showed a high susceptibility to ascorbate treatment, the combination treatment with FeCl3 generally led to a reduction in the ascorbate effect and in the formation of reactive oxygen species. The ascorbate‑induced cell death showed no signs of apoptosis but clear ferroptotic properties. Furthermore, treatment of the tumor cells with FeCl3 was accompanied by reduced expression of TfR1, preventing an increase in the intracellular labile iron pool. The present study provided valuable information on the mechanism of action of high‑dose vitamin C in pancreatic cancer, whereby a combination treatment with ferric iron in the context of tumor therapy is not recommended based on these data.