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Article
2021
Bacillus subtilis high cell density fermentation using a sporulation-deficient strain for the production of surfactin
Bacillus subtilis high cell density fermentation using a sporulation-deficient strain for the production of surfactin
Klausmann, Peter Hennemann, Katja Hoffmann, Mareen Treinen, Chantal Aschern, Moritz Lilge, Lars Morabbi Heravi, Kambiz Henkel, Marius Hausmann, Rudolf
Abstract (English)
Bacillus subtilis 3NA is a strain capable of reaching high cell densities. A surfactin producing sfp+ variant of this strain, named JABs32, was utilized in fed-batch cultivation processes. Both a glucose and an ammonia solution were fed to set a steady growth rate μ of 0.1 h-1. In this process, a cell dry weight of up to 88 g L-1 was reached after 38 h of cultivation, and surfactin titers of up to 26.5 g L-1 were detected in this high cell density fermentation process, achieving a YP/X value of 0.23 g g-1 as well as a qP/X of 0.007 g g-1 h-1. In sum, a 21-fold increase in surfactin titer was obtained compared with cultivations in shake flasks. In contrast to fed-batch operations using Bacillus subtilis JABs24, an sfp+ variant derived from B. subtilis 168, JABs32, reached an up to fourfold increase in surfactin titers using the same fed-batch protocol. Additionally, a two-stage feed process was established utilizing strain JABs32. Using an optimized mineral salt medium in this high cell density fermentation approach, after 31 h of cultivation, surfactin titers of 23.7 g L-1 were reached with a biomass concentration of 41.3 g L-1, thus achieving an enhanced YP/X value of 0.57 g g-1 as well as a qP/X of 0.018 g g-1 h-1. The mutation of spo0A locus and an elongation of AbrB in the strain utilized in combination with a high cell density fed-batch process represents a promising new route for future enhancements on surfactin production.
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Applied microbiology and biotechnology, 105 (2021), 10, 4141-4151.
https://doi.org/10.1007/s00253-021-11330-x.
ISSN: 1432-0614
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Klausmann, P., Hennemann, K., Hoffmann, M., Treinen, C., Aschern, M., Lilge, L., Morabbi Heravi, K., Henkel, M., & Hausmann, R. (2021). Bacillus subtilis high cell density fermentation using a sporulation-deficient strain for the production of surfactin. Applied microbiology and biotechnology, 105(10). https://doi.org/10.1007/s00253-021-11330-x
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660 Chemical engineerin
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Sustainable Development Goals
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@article{Klausmann2021,
url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16438},
doi = {10.1007/s00253-021-11330-x},
author = {Klausmann, Peter and Hennemann, Katja and Hoffmann, Mareen et al.},
title = {Bacillus subtilis High Cell Density Fermentation Using a Sporulation-Deficient Strain for the Production of Surfactin},
journal = {Applied microbiology and biotechnology},
year = {2021},
volume = {105},
number = {10},
}