NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis

dc.contributor.authorAlshareef, Nouf Owdah
dc.contributor.authorOtterbach, Sophie L.
dc.contributor.authorAllu, Annapurna Devi
dc.contributor.authorWoo, Yong H.
dc.contributor.authorde Werk, Tobias
dc.contributor.authorKamranfar, Iman
dc.contributor.authorMueller-Roeber, Bernd
dc.contributor.authorTester, Mark
dc.contributor.authorBalazadeh, Salma
dc.contributor.authorSchmöckel, Sandra M.
dc.contributor.corporateAlshareef, Nouf Owdah; Division of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
dc.contributor.corporateOtterbach, Sophie L.; Department Physiology of Yield Stability, Institute of Crop Science, University of Hohenheim, Stuttgart, Germany
dc.contributor.corporateAllu, Annapurna Devi; Department of Biology, Indian Institute of Science Education and Research (IISER), Tirupati, India
dc.contributor.corporateWoo, Yong H.; Division of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
dc.contributor.corporatede Werk, Tobias; Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany
dc.contributor.corporateKamranfar, Iman; Institute of Biochemistry and Biology, University of Potsdam, Potsdam‐Golm, Germany
dc.contributor.corporateMueller-Roeber, Bernd; Center of Plant Systems Biology and Biotechnology (CPSBB), Plovdiv, Bulgaria
dc.contributor.corporateTester, Mark; Division of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
dc.contributor.corporateBalazadeh, Salma; Institute of Biology, Leiden University, Leiden, The Netherlands
dc.contributor.corporateSchmöckel, Sandra M.; Department Physiology of Yield Stability, Institute of Crop Science, University of Hohenheim, Stuttgart, Germany
dc.date.accessioned2025-06-06T07:24:38Z
dc.date.available2025-06-06T07:24:38Z
dc.date.issued2022
dc.date.updated2024-12-02T06:33:05Z
dc.description.abstractPre-exposing (priming) plants to mild, non-lethal elevated temperature improves their tolerance to a later higher-temperature stress (triggering stimulus), which is of great ecological importance. ‘Thermomemory’ is maintaining this tolerance for an extended period of time. NAM/ATAF1/2/CUC2 (NAC) proteins are plant-specific transcription factors (TFs) that modulate responses to abiotic stresses, including heat stress (HS). Here, we investigated the potential role of NACs for thermomemory. We determined the expression of 104 Arabidopsis NAC genes after priming and triggering heat stimuli, and found ATAF1 expression is strongly induced right after priming and declines below control levels thereafter during thermorecovery. Knockout mutants of ATAF1 show better thermomemory than wild type, revealing a negative regulatory role. Differential expression analyses of RNA-seq data from ATAF1 overexpressor, ataf1 mutant and wild-type plants after heat priming revealed five genes that might be priming-associated direct targets of ATAF1: AT2G31260 (ATG9), AT2G41640 (GT61), AT3G44990 (XTH31), AT4G27720 and AT3G23540. Based on co-expression analyses applied to the aforementioned RNA-seq profiles, we identified ANAC055 to be transcriptionally co-regulated with ATAF1. Like ataf1, anac055 mutants show improved thermomemory, revealing a potential co-control of both NAC TFs over thermomemory. Our data reveals a core importance of two NAC transcription factors, ATAF1 and ANAC055, for thermomemory.en
dc.description.sponsorshipOpen Access funding enabled and organized by Projekt DEAL.
dc.description.sponsorshipL'Oréal-UNESCO For Women in Science 2014 Middle East Fellowship
dc.description.sponsorshipKing Abdullah University of Science and Technology (KAUST)
dc.description.sponsorshipInternational Max Planck Research School 'Primary Metabolism and Plant Growth'
dc.description.sponsorshipEuropean Union’s Horizon 2020 Research and Innovation Programme, project PlantaSYST
dc.description.sponsorshipUniversity of Potsdam
dc.description.sponsorshipMax Planck Institute of Molecular Plant Physiology
dc.description.sponsorshipMinistry for Science, Research and Art of Baden-Wuerttemberg
dc.description.sponsorshipUniversität Hohenheim (3153)
dc.identifier.swb1810693225
dc.identifier.urihttps://doi.org/10.1038/s41598-022-14429-x
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16964
dc.language.isoeng
dc.rights.licensecc_by
dc.subjectThermomemory
dc.subjectHeat stress tolerance
dc.subjectNAC transcription factors
dc.subjectArabidopsis
dc.subjectATAF1
dc.subjectGene expression
dc.subjectPriming
dc.subject.ddc630
dc.titleNAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsisen
dc.type.diniArticle
dcterms.bibliographicCitationScientific reports, 12 (2022), 11264. https://doi.org/10.1038/s41598-022-14429-x. ISSN: 2045-2322
dcterms.bibliographicCitation.issn2045-2322
dcterms.bibliographicCitation.issue1
dcterms.bibliographicCitation.journaltitleScientific reports
dcterms.bibliographicCitation.originalpublishernameNature Publishing Group UK
dcterms.bibliographicCitation.volume12
local.export.bibtex@article{Alshareef2022, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16964}, doi = {10.1038/s41598-022-14429-x}, author = {Alshareef, Nouf Owdah and Otterbach, Sophie L. and Allu, Annapurna Devi et al.}, title = {NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis}, journal = {Scientific reports}, year = {2022}, volume = {12}, }
local.export.bibtexAuthorAlshareef, Nouf Owdah and Otterbach, Sophie L. and Allu, Annapurna Devi et al.
local.export.bibtexKeyAlshareef2022
local.export.bibtexType@article
local.subject.sdg2
local.subject.sdg12
local.subject.sdg13
local.title.fullNAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis

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