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Abstract

Soil-borne fungal pathogens such as Sclerotium spp., Rhizoctonia spp., and Macrophomina spp. pose significant threats to global agriculture, with soybean crops among the most severely affected due to damping-off disease. These pathogens cause substantial yield losses, making their management a critical concern. In this study, we investigated the potential of Bacillus siamensis BCL, a novel Neotropical strain, as an eco-friendly solution for managing Sclerotium , Rhizoctonia , and Macrophomina species. The strain exhibited strong antifungal activity, significantly inhibiting fungal growth in vitro, with the greatest suppression observed against Macrophomina spp., reaching up to 81%. In vivo assays further confirmed the biocontrol potential of B. siamensis . When applied at 10 6 colony-forming units (CFU)/mL, the strain reduced disease symptoms and improved plant growth parameters—including root length, shoot biomass, and leaf number—compared to untreated, infected controls. The protective effect varied by pathogen, with the most significant recovery in root length observed against Macrophomina spp. (85%) and Sclerotium spp. (78%). In preventive treatments, fermentation extracts of the B. siamensis strain suppressed disease progression, although they did not promote seedling growth. A genomic analysis of B. siamensis BCL revealed genes encoding antimicrobial secondary metabolites, including terpenes, fengycins, and surfactins. These findings highlight B. siamensis BCL as a promising candidate for sustainable crop protection and a valuable resource for developing novel antimicrobial strategies in agriculture.

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Microorganisms, 13 (2025), 6, 1366. https://doi.org/10.3390/microorganisms13061366. ISSN: 2076-2607

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Moreira, R. F., Pires, E. B. E., Sousa, O. F., Alves, G. B., Viteri Jumbo, L. O., Santos, G. R., Maia, L. J., Ribeiro, B. M., Smagghe, G., Perino, E. H. B., Hausmann, R., Oliveira, E. E., & Aguiar, R. W. S. (2025). A Novel Neotropical Bacillus siamensis Strain Inhibits Soil-Borne Plant Pathogens and Promotes Soybean Growth. Microorganisms, 13(6). https://doi.org/10.3390/microorganisms13061366

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English

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630 Agriculture

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Sustainable Development Goals

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@article{Moreira2025, doi = {10.3390/microorganisms13061366}, author = {Moreira, Rodrigo F. and Pires, Elizabeth B. E. and Sousa, Odaiza F. et al.}, title = {A Novel Neotropical Bacillus siamensis Strain Inhibits Soil-Borne Plant Pathogens and Promotes Soybean Growth}, journal = {Microorganisms}, year = {2025}, volume = {13}, number = {6}, }

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