A new version of this entry is available:
Loading...
Article
2022
Online hyphenation of centrifugal partition chromatography with countercurrent chromatography (CPC-CCC) and its application to the separation of saturated alkylresorcinols
Online hyphenation of centrifugal partition chromatography with countercurrent chromatography (CPC-CCC) and its application to the separation of saturated alkylresorcinols
Abstract (English)
Centrifugal partition chromatography (CPC) and countercurrent chromatography (CCC) are two preparative techniques mainly used for the isolation and purification of natural products. While CPC benefits from a larger sample capacity, CCC typically provides better peak resolutions and hereby higher purities. In this study, we aimed to combine both advantages by the direct linking of CPC and CCC which was achieved by installation of switching valves and connection tube. The hyphenated CPC-CCC setup was tested with major alkylresorcinols which were obtained from a transesterified and hydrogenated rye extract. Injections of 1- and 5-g samples into the individual CCC system confirmed the limited sample capacity because of immediate flooding with the 5-g sample (total loss of stationary phase). In comparison, the CPC system was stable with 5- and 10-g samples but the peak resolution with 1-g sample was poorer than with the CCC system. Injections of 5- and 10-g samples into the CPC-CCC system were successful. However, a sample load of 10 g resulted in lower purities of the alkylresorcinols (80% or less) due to peak tailing. By contrast, injection of 5-g sample provided high amounts of ~ 1.2 g alkylresorcinols with purities of > 95%.
File is subject to an embargo until
This is a correction to:
A correction to this entry is available:
This is a new version of:
Other version
Notes
Publication license
Publication series
Published in
Analytical and bioanalytical chemistry, 414 (2022), 17, 5043-5051.
https://doi.org/10.1007/s00216-022-04136-x.
ISSN: 1618-2650
Other version
Faculty
Institute
Examination date
Supervisor
Cite this publication
Hammerschick, T., & Vetter, W. (2022). Online hyphenation of centrifugal partition chromatography with countercurrent chromatography (CPC-CCC) and its application to the separation of saturated alkylresorcinols. Analytical and bioanalytical chemistry, 414(17). https://doi.org/10.1007/s00216-022-04136-x
Edition / version
Citation
DOI
ISSN
ISBN
Language
English
Publisher
Publisher place
Classification (DDC)
540 Chemistry
Collections
Original object
University bibliography
Standardized keywords (GND)
Sustainable Development Goals
BibTeX
@article{Hammerschick2022,
doi = {10.1007/s00216-022-04136-x},
author = {Hammerschick, Tim and Vetter, Walter},
title = {Online hyphenation of centrifugal partition chromatography with countercurrent chromatography (CPC-CCC) and its application to the separation of saturated alkylresorcinols},
journal = {Analytical and Bioanalytical Chemistry},
year = {2022},
volume = {414},
number = {17},
pages = {5043--5051},
}