Comparison of aqueous and lactobacterial-fermented Mercurialis perennis L. (Dog’s Mercury) extracts with respect to their immunostimulating activity

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dc.contributor.authorLorenz, Peter
dc.contributor.authorZilkowski, Ilona
dc.contributor.authorMailänder, Lilo K.
dc.contributor.authorKlaiber, Iris
dc.contributor.authorNicolay, Sven
dc.contributor.authorGarcia-Käufer, Manuel
dc.contributor.authorZimmermann-Klemd, Amy M.
dc.contributor.authorTurek, Claudia
dc.contributor.authorStintzing, Florian C.
dc.contributor.authorKammerer, Dietmar R.
dc.contributor.authorGründemann, Carsten
dc.date.accessioned2024-09-03T13:38:01Z
dc.date.available2024-09-03T13:38:01Z
dc.date.issued2023de
dc.description.abstractLactic acid (LA) fermentation of dog’s mercury (M. perennis L.) herbal parts was investigated in samples inoculated with either Lactobacteria (Lactobacillus plantarum and Pediococcus pentosaceus, LBF) or whey (WF). Depending on fermentation time, LA concentrations were monitored in a range of 3.4–15.6 g/L with a concomitant pH decline from 6.5 to 3.9. A broad spectrum of cinnamic acids depsides containing glucaric, malic and 2-hydroxyglutaric acids along with quercetin and kaempferol glycosides were detected by LC-DAD-ESI-MSn. Moreover, in this study novel constituents were also found both in unfermented and fermented extracts. Furthermore, amino acids and particular Lactobacteria metabolites such as biogenic amines (e.g., putrescine, 4-aminobutyric acid, cadaverine) and 5-oxoproline were assigned in WF extracts by GC-MS analysis after silylation. Enhanced NFκB and cytokine expression (IL-6, TNFα, IL-8 and IL-1β) was induced by all extracts, both non-fermented and fermented, in NFκB-THP-1 reporter cells, showing a concentration-dependent immunostimulatory effect. The WF extracts were tested for micronuclei formation in THP-1 cells and toxicity in luminescent bacteria (V. fischeri), whereby no mutagenic or toxic effects could be detected, which corroborates their safe use in pharmaceutical remedies.en
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16493
dc.identifier.urihttps://doi.org/10.3390/fermentation9020190
dc.language.isoengde
dc.rights.licensecc_byde
dc.source2311-5637de
dc.sourceFermentation; Vol. 9, No. 2 (2023) 190de
dc.subjectM. perennis
dc.subjectLactic acid fermentation
dc.subjectLactobacillus
dc.subjectPediococcus
dc.subjectDepsides
dc.subjectFlavonoids
dc.subjectCytokine expression
dc.subjectImmunostimulation
dc.subjectGenotoxicity
dc.subject.ddc610
dc.titleComparison of aqueous and lactobacterial-fermented Mercurialis perennis L. (Dog’s Mercury) extracts with respect to their immunostimulating activityen
dc.type.diniArticle
dcterms.bibliographicCitationFermentation, 9 (2023), 2, 190. https://doi.org/10.3390/fermentation9020190. ISSN: 2311-5637
dcterms.bibliographicCitation.issn2311-5637
dcterms.bibliographicCitation.issue2
dcterms.bibliographicCitation.journaltitleFermentation
dcterms.bibliographicCitation.volume9
local.export.bibtex@article{Lorenz2023, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16493}, doi = {10.3390/fermentation9020190}, author = {Lorenz, Peter and Zilkowski, Ilona and Mailänder, Lilo K. et al.}, title = {Comparison of aqueous and lactobacterial-fermented Mercurialis perennis L. (Dog’s Mercury) extracts with respect to their immunostimulating activity}, journal = {Fermentation}, year = {2023}, volume = {9}, number = {2}, }
local.export.bibtexAuthorLorenz, Peter and Zilkowski, Ilona and Mailänder, Lilo K. et al.
local.export.bibtexKeyLorenz2023
local.export.bibtexType@article

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