Successful silencing of the mycotoxin synthesis gene TRI5 in fusarium culmorum and observation of reduced virulence in VIGS and SIGS experiments

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dc.contributor.authorTretiakova, Polina
dc.contributor.authorVoegele, Ralf Thomas
dc.contributor.authorSoloviev, Alexander
dc.contributor.authorLink, Tobias Immanuel
dc.date.accessioned2024-10-23T12:25:52Z
dc.date.available2024-10-23T12:25:52Z
dc.date.issued2022de
dc.description.abstractCrops constantly experience various biotic stresses during their life cycle, and Fusarium spp. remain one of the most serious groups of pathogens affecting plants. The ability to manipulate the expression of certain microorganism genes via RNAi creates the opportunity for new-generation dsRNA-based preparations to control a large number of diseases. In this study, we applied virus-induced gene silencing (VIGS), and spray-induced gene silencing (SIGS) to silence the trichothecene-producing gene TRI5 in F. culmorum as a means to reduce its aggressiveness on spring wheat. Treatment of the fungus with dsTRI5RNA in vitro reduced deoxynivalenol (DON) and 3-acetyldeoxynivalenol (3-A-DON) accumulations by 53–85% and 61–87%, respectively, and reduced TRI5 expression by 84–97%. VIGS decreased the proportion of infected wheat spikelets by 73%, but upregulation was observed for TRI5. SIGS on wheat leaves and ears using certain dsTRI5RNA amounts negatively impacted F. culmorum growth. However, when performing in vivo analyses of TRI5 mRNA levels, the upregulation of the gene was determined in the variants where fungal colonization was restricted, suggesting a compensatory reaction of the pathogen to RNAi.en
dc.identifier.swb1796241113
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16817
dc.identifier.urihttps://doi.org/10.3390/genes13030395
dc.language.isoengde
dc.rights.licensecc_byde
dc.source2073-4425de
dc.sourceGenes; Vol. 13, No. 3 (2022) 395de
dc.subjectF. culmorum´
dc.subjectFHB
dc.subjectdsRNA
dc.subjectRNAi
dc.subjectVIGS
dc.subjectSIGS
dc.subjectTrichothecenes
dc.subjectVirulence
dc.subjectGene expression
dc.subject.ddc630
dc.titleSuccessful silencing of the mycotoxin synthesis gene TRI5 in fusarium culmorum and observation of reduced virulence in VIGS and SIGS experimentsen
dc.type.diniArticle
dcterms.bibliographicCitationGenes, 13 (2022), 3, 395. https://doi.org/10.3390/genes13030395. ISSN: 2073-4425
dcterms.bibliographicCitation.issn2073-4425
dcterms.bibliographicCitation.issue3
dcterms.bibliographicCitation.journaltitleGenes
dcterms.bibliographicCitation.volume13
local.export.bibtex@article{Tretiakova2022, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16817}, doi = {10.3390/genes13030395}, author = {Tretiakova, Polina and Voegele, Ralf Thomas and Soloviev, Alexander et al.}, title = {Successful Silencing of the Mycotoxin Synthesis Gene TRI5 in Fusarium culmorum and Observation of Reduced Virulence in VIGS and SIGS Experiments}, journal = {Genes}, year = {2022}, volume = {13}, number = {3}, }
local.export.bibtexAuthorTretiakova, Polina and Voegele, Ralf Thomas and Soloviev, Alexander et al.
local.export.bibtexKeyTretiakova2022
local.export.bibtexType@article

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