Loss of LaMATE impairs isoflavonoid release from cluster roots of phosphorus‐deficient white lupin

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dc.contributor.authorZhou, Yaping
dc.contributor.authorOlt, Philipp
dc.contributor.authorNeuhäuser, Benjamin
dc.contributor.authorMoradtalab, Narges
dc.contributor.authorBautista, William
dc.contributor.authorUhde‐Stone, Claudia
dc.contributor.authorNeumann, Günter
dc.contributor.authorLudewig, Uwe
dc.date.accessioned2024-11-06T10:17:37Z
dc.date.available2024-11-06T10:17:37Z
dc.date.issued2021de
dc.description.abstractWhite lupin (Lupinus albus L.) forms brush‐like root structures called cluster roots under phosphorus‐deficient conditions. Clusters secrete citrate and other organic compounds to mobilize sparingly soluble soil phosphates. In the context of aluminum toxicity tolerance mechanisms in other species, citrate is released via a subgroup of MATE/DTX proteins (multidrug and toxic compound extrusion/detoxification). White lupin contains 56 MATE/DTX genes. Many of these are closely related to gene orthologs with known substrates in other species. LaMATE is a marker gene for functional, mature clusters and is, together with its close homolog LaMATE3, a candidate for the citrate release. Both were highest expressed in mature clusters and when expressed in oocytes, induced inward‐rectifying currents that were likely carried by endogenous channels. No citrate efflux was associated with LaMATE and LaMATE3 expression in oocytes. Furthermore, citrate secretion was largely unaffected in P‐deficient composite mutant plants with genome‐edited or RNAi‐silenced LaMATE in roots. Moderately lower concentrations of citrate and malate in the root tissue and consequently less organic acid anion secretion and lower malate in the xylem sap were identified. Interestingly, however, less genistein was consistently found in mutant exudates, opening the possibility that LaMATE is involved in isoflavonoid release.en
dc.identifier.swb1765844312
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16889
dc.identifier.urihttps://doi.org/10.1111/ppl.13515
dc.language.isoengde
dc.rights.licensecc_byde
dc.source1399-3054de
dc.sourcePhysiologia plantarum; Vol. 173, No. 3 (2021), 1207-1220de
dc.subjectWhite lupin (Lupinus albus)
dc.subjectCluster roots
dc.subjectPhosphorus deficiency
dc.subjectCitrate secretion
dc.subjectMATE/DTX proteins
dc.subjectAluminum toxicity tolerance
dc.subjectLaMATE gene
dc.subjectOrganic acid anion secretion
dc.subjectGenome editing (RNAi silencing)
dc.subjectIsoflavonoid release
dc.subject.ddc580
dc.titleLoss of LaMATE impairs isoflavonoid release from cluster roots of phosphorus‐deficient white lupinen
dc.type.diniArticle
dcterms.bibliographicCitationPhysiologia plantarum, 173 (2021), 3, 1207-1220. https://doi.org/10.1111/ppl.13515. ISSN: 1399-3054
dcterms.bibliographicCitation.issn1399-3054
dcterms.bibliographicCitation.issue3
dcterms.bibliographicCitation.journaltitlePhysiologia plantarum
dcterms.bibliographicCitation.volume173
local.export.bibtex@article{Zhou2021, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16889}, doi = {10.1111/ppl.13515}, author = {Zhou, Yaping and Olt, Philipp and Neuhäuser, Benjamin et al.}, title = {Loss of LaMATE impairs isoflavonoid release from cluster roots of phosphorus‐deficient white lupin}, journal = {Physiologia plantarum}, year = {2021}, }
local.export.bibtexAuthorZhou, Yaping and Olt, Philipp and Neuhäuser, Benjamin et al.
local.export.bibtexKeyZhou2021
local.export.bibtexType@article

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