A new version of this entry is available:
Loading...
Article
2024
Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat
Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat
Abstract (English)
Cultured or cultivated meat, animal muscle, and fat tissue grown in vitro, could transform the global meat market, reducing animal suffering while using fewer resources than traditional meat production and no antimicrobials at all. To ensure the appeal of cultured meat to future customers, cultured fat is essential for achieving desired mouthfeel, taste, and texture, especially in beef. In this work we show the establishment of primary bovine adipose-derived stem cell spheroids in static and dynamic suspension culture. Spheroids are successfully differentiated using a single-step protocol. Differentiated spheroids from dynamic cultures maintain stability and viability during 3D bioprinting in edible gellan gum. Also, the fatty acid composition of differentiated spheroids is significantly different from control spheroids. The cells are cultured antibiotic-free to minimize the use of harmful substances. This work presents a stable and bioprintable building block for cultured fat with a high cell density in a 3D dynamic cell culture system.
File is subject to an embargo until
This is a correction to:
A correction to this entry is available:
This is a new version of:
Other version
Notes
Publication license
Publication series
Published in
Nature Communications, 15 (2024), 1, 9107.
https://doi.org/10.1038/s41467-024-53486-w.
ISSN: 2041-1723
London : Nature Publishing Group UK
Other version
Faculty
Institute
Examination date
Supervisor
Cite this publication
Klatt, A., Wollschlaeger, J. O., Albrecht, F. B., Rühle, S., Holzwarth, L. B., Hrenn, H., Melzer, T., Heine, S., & Kluger, P. J. (2024). Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat. Nature Communications, 15(1). https://doi.org/10.1038/s41467-024-53486-w
Edition / version
Citation
DOI
ISSN
ISBN
Language
English
Publisher
Publisher place
Classification (DDC)
660 Chemical engineerin
Collections
Original object
University bibliography
Free keywords
Standardized keywords (GND)
Sustainable Development Goals
BibTeX
@article{Klatt2024,
doi = {10.1038/s41467-024-53486-w},
author = {Klatt, Annemarie and Wollschlaeger, Jannis O. and Albrecht, Franziska B. et al.},
title = {Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat},
journal = {Nature Communications},
year = {2024},
volume = {15},
number = {1},
}