A new version of this entry is available:
Loading...
Article
2024
Capsaicin attenuates the effect of inflammatory cytokines in a HaCaT cell model for basal keratinocytes
Capsaicin attenuates the effect of inflammatory cytokines in a HaCaT cell model for basal keratinocytes
Abstract (English)
Introduction: The resolution of the skin’s inflammatory response is only possible if its barrier function is restored. TRPV1 channel activation plays an important role during inflammation but the effect of this activation on the skin barrier under inflammatory conditions has not been clarified. We hypothesize that it could potentially aid the keratinocyte barrier by reducing inflammatory cytokine release and promoting tight junction development. Methods: To explore the role of TRPV1 activation in inflammation, we designed and optimized an in vitro model of keratinocytes with basal epidermal layer characteristics using HaCaT cells and TNFα to induce inflammation. Results: TNFα increased the gene expression of tight junction protein claudin 1 (CLDN1) by at least 2.60 ± 0.16-fold, in a concentration-dependent manner, over a 48 h period. The administration of a capsaicin pre-treatment reduced the CLDN1 expression to 1.51 ± 0.16-fold during the first 6 h after TNFα induction, whereas IL-8 cytokine release was reduced 0.64 ± 0.17-fold. After 48 h, CLDN1 protein levels increased by a factor of 6.57 ± 1.39 compared to cells only treated with TNFα. Discussion: These results suggest that activation of TRPV1 by capsaicin can potentiate the increase in CLDN1 expression and CLDN1 protein synthesis induced by TNFα in cultured keratinocytes, while reducing the release of IL-8.
File is subject to an embargo until
This is a correction to:
A correction to this entry is available:
This is a new version of:
Other version
Notes
Publication license
Publication series
Published in
Frontiers in pharmacology, 15 (2024), 1474898.
https://doi.org/10.3389/fphar.2024.1474898.
ISSN: 1663-9812
Other version
Faculty
Institute
Examination date
Supervisor
Cite this publication
Cervantes Recalde, M. F., Schmidt, J., Girardi, C., Massironi, M., Rechl, M. L., Hans, J., Stuhlmann, D., Somoza, V., & Lieder, B. (2024). Capsaicin attenuates the effect of inflammatory cytokines in a HaCaT cell model for basal keratinocytes. Frontiers in pharmacology, 15. https://doi.org/10.3389/fphar.2024.1474898
Edition / version
Citation
DOI
ISSN
ISBN
Language
English
Publisher
Publisher place
Classification (DDC)
610 Medicine and health
Collections
Original object
University bibliography
Free keywords
Standardized keywords (GND)
Sustainable Development Goals
BibTeX
@article{Cervantes Recalde2024,
doi = {10.3389/fphar.2024.1474898},
author = {Cervantes Recalde, Maria Fernanda and Schmidt, Jana and Girardi, Cristina et al.},
title = {Capsaicin attenuates the effect of inflammatory cytokines in a HaCaT cell model for basal keratinocytes},
journal = {Frontiers in Pharmacology},
year = {2024},
volume = {15},
}