Institut für Ernährungswissenschaften

Permanent URI for this collectionhttps://hohpublica.uni-hohenheim.de/handle/123456789/5

Browse

Browsing Institut für Ernährungswissenschaften by Journal "Antioxidants"

Type the first few letters and click on the Browse button
Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    An in vitro and in silico study of antioxidant properties of curcuminoid N-alkylpyridinium salts: Initial assessment of their antitumoral properties
    (2022) Forero-Doria, Oscar; Guzmán, Luis; Jiménez-Aspee, Felipe; Echeverría, Javier; Wehinger, Sergio; Valenzuela, Claudio; Araya-Maturana, Ramiro; Martínez-Cifuentes, Maximiliano
    In this work, we report the synthesis of curcuminoids with ionic liquid characteristics, obtained by incorporating alkyl-substituted pyridinium moiety rather than one phenyl group through a two-step process. The antioxidant capacity of the obtained compounds was evaluated in vitro by 1,1-diphenyl-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) assays, showing that some derivatives are more potent than curcumin. Pyridine curcuminoids (group 4) and curcuminoid N-alkylpyridinium salts with two methoxyl groups in the phenyl ring (group 7), presented the best antioxidant capacity. The experimental results were rationalized by density functional theory (DFT) calculations of the bond dissociation enthalpy (BDE) for O–H in each compound. The computational calculations allowed for insight into the structural–antioxidant properties relationship in these series of compounds. BDEs, obtained in the gas phase and water, showed a notable impact of water solvation on the stabilization of some radicals. The lower values of BDEs in the water solution correspond to the structurally related compounds curcuminoid-pyridine 4c and curcuminoid pyridinium salt 7a, which is consistent with the experimental results. Additionally, an assessment of cell viability and cell migration assays was performed for human colon cancer (HT29), human breast cancer (MCF7) cells, in addition to NIH3T3 murine fibroblast, as a model of non-cancer cell type. These compounds mainly cause inhibition of the cell migration observed in MCF7 cancer cells without affecting the non-tumoral NIH3T3 cell line: Neither in viability nor in migration.
  • Thumbnail Image
    Publication
    In vitro maturation of bovine oocytes in the presence of resveratrol and ellagic acid but not chlorogenic acid modulates blastocyst antioxidant gene expression without impacting embryo development and oxygen consumption
    (2025) Giller, Katrin; Schmid, Dominique; Serbetci, Idil; Meleán, Manuel; Greve, Sarah; von Meyenn, Ferdinand; Bollwein, Heinrich; Herrera, Carolina; Giller, Katrin; Department of Molecular Nutritional Science, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany;; Schmid, Dominique; Animal Nutrition, ETH Zurich, Universitaetstrasse 2, 8092 Zurich, Switzerland; Serbetci, Idil; AgroVet-Strickhof, University of Zurich, Eschikon 27, 8315 Lindau, Switzerland; (I.S.); (M.M.); (H.B.); (C.H.); Meleán, Manuel; AgroVet-Strickhof, University of Zurich, Eschikon 27, 8315 Lindau, Switzerland; (I.S.); (M.M.); (H.B.); (C.H.); Greve, Sarah; Department of Molecular Nutritional Science, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany;; von Meyenn, Ferdinand; Laboratory of Nutrition and Metabolic Epigenetics, ETH Zurich, Schorenstrasse 16, 8603 Schwerzenbach, Switzerland;; Bollwein, Heinrich; AgroVet-Strickhof, University of Zurich, Eschikon 27, 8315 Lindau, Switzerland; (I.S.); (M.M.); (H.B.); (C.H.); Herrera, Carolina; AgroVet-Strickhof, University of Zurich, Eschikon 27, 8315 Lindau, Switzerland; (I.S.); (M.M.); (H.B.); (C.H.); Jiang, Zhongliang
    In vitro fertilization is used to produce embryos from high-genetic-merit cattle. However, these embryos often exhibit inferior quality than those derived in vivo, possibly due to increased oxidative stress. This study investigates whether adding antioxidant polyphenols (resveratrol (RV), chlorogenic acid (CA), ellagic acid (EA)) to the in vitro maturation (IVM) medium at 0.25, 0.5, and 1 μM could improve embryo development. Oxygen consumption and gene expression were evaluated at the blastocyst stage following treatment with 1 μM of each polyphenol. Embryo development (cleavage, blastocyst, and hatched blastocyst rates) and oxygen consumption were not significantly affected by polyphenols. However, RV significantly upregulated the mRNA expression of the antioxidant enzyme glutathione peroxidase 4 (GPX4), while GPX4 expression was significantly downregulated by EA. Expression of other gene markers related to antioxidant defense, apoptosis, development, and metabolism was not significantly affected. The results indicate that applying RV, CA, and EA during bovine oocyte IVM does not enhance in vitro embryo development at the tested concentrations. Given the opposing effects of RV and EA on the expression of GPX4, the effects of those polyphenols regarding the protection of embryos from oxidative stress and potential long-term effects on the offspring remain to be elucidated.