Browsing by Person "Lieder, Barbara"

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Capsaicin attenuates the effect of inflammatory cytokines in a HaCaT cell model for basal keratinocytes
(2024) Cervantes Recalde, Maria Fernanda; Schmidt, Jana; Girardi, Cristina; Massironi, Marco; Rechl, Markus Leo; Hans, Joachim; Stuhlmann, Dominik; Somoza, Veronika; Lieder, Barbara
Introduction: The resolution of the skin’s inflammatory response is only possible if its barrier function is restored. TRPV1 channel activation plays an important role during inflammation but the effect of this activation on the skin barrier under inflammatory conditions has not been clarified. We hypothesize that it could potentially aid the keratinocyte barrier by reducing inflammatory cytokine release and promoting tight junction development. Methods: To explore the role of TRPV1 activation in inflammation, we designed and optimized an in vitro model of keratinocytes with basal epidermal layer characteristics using HaCaT cells and TNFα to induce inflammation. Results: TNFα increased the gene expression of tight junction protein claudin 1 (CLDN1) by at least 2.60 ± 0.16-fold, in a concentration-dependent manner, over a 48 h period. The administration of a capsaicin pre-treatment reduced the CLDN1 expression to 1.51 ± 0.16-fold during the first 6 h after TNFα induction, whereas IL-8 cytokine release was reduced 0.64 ± 0.17-fold. After 48 h, CLDN1 protein levels increased by a factor of 6.57 ± 1.39 compared to cells only treated with TNFα. Discussion: These results suggest that activation of TRPV1 by capsaicin can potentiate the increase in CLDN1 expression and CLDN1 protein synthesis induced by TNFα in cultured keratinocytes, while reducing the release of IL-8.
A high sucrose detection threshold is associated with increased energy intake and improved post-prandial glucose response independent of the sweetness intensity of isocaloric sucrose solutions
(2024) Preinfalk, Verena; Schweiger, Kerstin; Hüller, Leonie; Dunkel, Andreas; Kimmeswenger, Isabella; Deck, Corinna M.; Rust, Petra; Somoza, Veronika; Krammer, Gerhard E.; Ley, Jakob P.; Lieder, Barbara
Several studies proposed a role for the sweet taste receptor in energy intake and blood glucose regulation, but little is yet known about the impact of the individual sweet taste perception. Here, we found in a cross-over human intervention study with 29 male participants that modulating the sweetness of an isocaloric sucrose solution did not influence postprandial plasma concentrations of blood glucose and associated hormones over 120 min and 2 h post-load energy intake. Independent of the sweetness of the test solution, tests persons with a higher sucrose detection threshold had an average of 402 ± 78.8 kcal (39 ± 21%) higher energy intake and a higher glucose/insulin ratio, combined with a higher liking for sweet tasting food, than the test persons of the low threshold group. The body composition suggested a higher fat-free mass in the high threshold group that may have influenced energy intake and post-prandial glucose responses.
Impaired metal perception and regulation of associated human foliate papillae tongue transcriptome in long-COVID-19
(2024) Danzer, Barbara; Jukic, Mateo; Dunkel, Andreas; Andersen, Gaby; Lieder, Barbara; Schaudy, Erika; Stadlmayr, Sarah; Lietard, Jory; Michel, Timm; Krautwurst, Dietmar; Haller, Bernhard; Knolle, Percy; Somoza, Mark; Lingor, Paul; Somoza, Veronika; Danzer, Barbara; School of Life Science, Technical University of Munich, Freising, Germany; Jukic, Mateo; Department of Neurology, School of Medicine and Health, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany; Dunkel, Andreas; Leibniz Institute for Food Systems Biology at the Technical University of Munich, Freising, Germany; Andersen, Gaby; Leibniz Institute for Food Systems Biology at the Technical University of Munich, Freising, Germany; Lieder, Barbara; Department of Physiological Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria; Schaudy, Erika; Department of Inorganic Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria; Stadlmayr, Sarah; Department of Physiological Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria; Lietard, Jory; Department of Inorganic Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria; Michel, Timm; School of Life Science, Technical University of Munich, Freising, Germany; Krautwurst, Dietmar; Leibniz Institute for Food Systems Biology at the Technical University of Munich, Freising, Germany; Haller, Bernhard; Institute of AI and Informatics in Medicine, School of Medicine and Health, Technical University of Munich, Munich, Germany; Knolle, Percy; Institute of Molecular Immunology, School of Medicine and Health, Technical University of Munich, Munich, Germany; Somoza, Mark; Leibniz Institute for Food Systems Biology at the Technical University of Munich, Freising, Germany; Lingor, Paul; Department of Neurology, School of Medicine and Health, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany; Somoza, Veronika; Leibniz Institute for Food Systems Biology at the Technical University of Munich, Freising, Germany
Chemosensory impairment is an outstanding symptom of SARS-CoV-2 infections. We hypothesized that measured sensory impairments are accompanied by transcriptomic changes in the foliate papillae area of the tongue. Hospital personnel with known SARS-CoV-2 immunoglobulin G (IgG) status completed questionnaires on sensory perception ( n = 158). A subcohort of n = 141 participated in forced choice taste tests, and n = 43 participants consented to donate tongue swabs of the foliate papillae area for whole transcriptome analysis. The study included four groups of participants differing in IgG levels (≥ 10 AU/mL = IgG + ; < 10 AU/mL = IgG - ) and self-reported sensory impairment (SSI ± ). IgG + subjects not detecting metallic taste had higher IgG + levels than IgG + participants detecting iron gluconate ( p = 0.03). Smell perception was the most impaired biological process in the transcriptome data from IgG + /SSI + participants subjected to gene ontology enrichment. IgG + /SSI + subjects demonstrated lower expression levels of 166 olfactory receptors (OR) and 9 taste associated receptors (TAS) of which OR1A2, OR2J2, OR1A1, OR5K1 and OR1G1, as well as TAS2R7 are linked to metallic perception. The question raised by this study is whether odorant receptors on the tongue (i) might play a role in metal sensation, and (ii) are potential targets for virus-initiated sensory impairments, which needs to be investigated in future functional studies.
Role of homovanillic acid esters in the regulation of skin inflammatory pathways and their effect on tight junction protein expression
(2025) Cervantes Recalde, Maria Fernanda; Bogensperger, Elena Zoe; Hans, Joachim; Stuhlmann, Dominik; Somoza, Veronika; Lieder, Barbara
In the context of epidermal inflammation, the inflammatory response not only involves the release of inflammatory cytokines like interleukin 8 (IL-8), but also modulation of tight junction protein expression levels. Previous studies showed that the tight junction protein claudin 1 (CLDN1) is upregulated during tumor necrosis factor α (TNFα)-induced inflammation by capsaicin in keratinocytes in a transient receptor potential channel vanilloid 1 (TRPV1)-dependent manner. However, the caveat with TRPV1 ligands is the undesired pain response elicited by the activation of neuronal TRPV1 channels. In this study, we hypothesized that also less or non-pungent homovanillic acid esters as structural analogs of capsaicin target CLDN1 upregulation during inflammation. Methods: We aimed to identify beneficial structural characteristics by selecting homovanillic acid esters with different aliphatic tail structures and screening them for CLDN1 upregulation at early stages of TNFα-induced inflammation in basal keratinocytes. Results: CLDN1 expression was upregulated independently of TRPV1 by compounds with a tail of 5 or 6 C-atoms, regardless of the presence of ramifications and double bonds with a maximum fold change of 2.05 ± 0.22 against control. The induction of CLDN1 expression was accompanied by increased expression of the differentiation marker involucrin (IVL). Discussion: The results suggest that the homovanillic ester-induced CLDN1 upregulation is a result of increased differentiation of the basal keratinocytes towards the keratinocyte morphology present in the stratum granulosum (SG), where tight junctions are formed. In conclusion, homovanillic acid esters with a 5 or 6 C-atom long aliphatic chain induced CLDN1 expression, thereby stimulating keratinocyte differentiation, independent from TRPV1 activation.