Publikationsfonds der Universität Hohenheim

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Über den Publikationsfonds der Universität Hohenheim erhalten Wissenschaftlerinnen und Wissenschaftler der Universität finanzielle Unterstützung bei der Veröffentlichung ihrer Forschungsergebnisse im Open Access. Gefördert werden Zeitschriftenartikel in Fully-Open-Access-Zeitschriften (Gold-OA) und hybriden Subskriptionszeitschriften (Hybrid-OA) sowie Monografien. Autorinnen und Autoren können online einen Förderantrag zur Finanzierungsbeteiligung ihrer Publikation stellen.

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Effects of dietary phosphorus and myo-inositol supplementation on NaPi-IIb and TRPV6 protein expression in duodenal apical membranes of laying hens from two strains
(2026) Shomina, Nataliia; Sommerfeld, Vera; Hanauska, Anna; Oster, Michael; Rodehutscord, Markus; Huber, Korinna
Phosphorus (P) and calcium (Ca) absorption in the intestine is mediated by apical brush border membrane (BBM) transporters, including the sodium-dependent phosphate (Pi) transporter NaPi-IIb and the Ca²⁺-selective channel TRPV6. Both are highly expressed in the duodenum and exhibit dietary adaptability; yet little is known about how this adaptability varies with strain and age in laying hens. The present study examined the effects of dietary mineral P renunciation and myo-inositol (MI) supplementation on NaPi-IIb and TRPV6 protein expression in the duodenal BBM of Lohmann Brown-Classic (LB) and Lohmann LSL-Classic (LSL) hens. Two independent feeding trials were conducted: hens received diets either with or without mineral P supplementation (wk 15 - 19 and 20 – 24), or with graded MI levels (0 - 3 g/kg; wk 26 – 30). At the end of each period, hens were euthanized and protein expression of NaPi-IIb and TRPV6 in duodenal BBM was studied by western blotting. Statistical correlation with additional traits of mineral metabolism was analyzed. An immunoreactive NaPi-IIb band was detected at ∼ 45 kDa; therefore, all results reported here refer to this NaPi-IIb fragment. Mineral P renunciation did not affect NaPi-IIb fragment or TRPV6 expression in either hen strain. In LSL hens NaPi-IIb fragment expression increased from wk 19 to wk 24, whereas in LB hens it remained unchanged. NaPi-IIb fragment expression was positively associated with duodenal phosphatase activity and plasma estradiol. TRPV6 expression tended to reduce in LB hens from wk 19 to wk 24, but remained stable in LSL hens. TRPV6 expression was positively associated with duodeno-jejunal P content. MI supplementation upregulated NaPi-IIb fragment expression in LB, but downregulated it in LSL hens with high dietary MI levels, without affecting TRPV6. These findings demonstrate strain-dependent regulatory patterns of duodenal expression of NaPi-IIb fragment and TRPV6 in response to physiological stage and MI supply, indicating that mineral feeding strategies may benefit from genotype-specific consideration, whereas the mechanisms underlying MI-related effects require further clarification.
Workplace stressors and burnout among healthcare professionals: Insights from the pandemic and implications for future public health crises
(2025) Daneshvar, Elahe; Otterbach, Steffen
This study evaluates the prevalence of burnout among healthcare professionals (HCPs) during the COVID-19 pandemic in Iran and examines its association with key occupational stressors (workload, job control, and leadership communication). Furthermore, it proposes relevant organisational interventions for future pandemic preparedness. A cross-sectional survey of HCPs (N = 723) was conducted in four hospitals in Tehran during the third peak of the COVID-19 pandemic in Iran, including the Copenhagen Burnout Inventory and items on perceived workload, job control, and leadership communication. The study found that 67.41% of HCPs reported substantial symptoms of burnout (moderate-to-high burnout, i.e. CBI ≥ 50), with a mean score of 59.6 points. The prevalence of burnout (CBI ≥ 50) was prominent across all three dimensions—personal, work, and patient-related—at 72.86%, 69.87%, and 65.37%, respectively. The analysis demonstrated significant associations between burnout and the three foundational workplace stressors. Multivariate linear regression analysis showed that frontline, female, and married HCPs reported the highest levels of burnout. This study provides practical implications for healthcare organisations and policy makers, highlighting the need for targeted organizational interventions that could mitigate burnout during ongoing and future health crises.
Nutrient–response modeling with a single and interpretable artificial neuron
(2025) Rodehutscord, Markus; Ahmadi, Hamed
Precise estimation of nutrient requirements and utilization efficiency is fundamental to nutritional sciences, yet it is mainly performed using classical nonlinear regression models. These models are interpretable but require careful selection of the functional form and initial parameter values. Flexible machine learning (ML) methods are seemingly disliked due to their perceived “black box” nature, which can obscure biological insight. A minimal and interpretable ML framework addresses this gap in nutrient–response modeling. The proposed approach uses a single artificial neuron with a hyperbolic tangent activation. Mathematically, this resembles a four-parameter sigmoidal function but with greater flexibility and distinct parameter definitions, allowing capture of the monotonic, saturating dynamics typical of essential nutrient responses. The method is enhanced with modern ML best practices, including data augmentation, Bayesian regularization, and bootstrap resampling, providing robust, uncertainty-quantified estimates of key nutritional metrics—such as asymptotic response, inflection point, and nutrient requirements—even from small datasets. Evaluations across 12 diverse datasets from poultry and fish studies, including amino acids and phosphorus, demonstrated that the single artificial neuron matches or exceeds the performance of classical models while providing full analytical transparency. The framework is implemented as a no-code graphical application, ‘NutriCurvist’, offering an easy-to-use alternative tool for nutrient-response modeling to support data-driven, precision nutrition.
Comparing hops and malt price transmission in the beer value chain: evidence from Germany
(2025) Hess, Sebastian; Bublik, Nikolas
The German beer value chain has received limited attention so far, despite the country’s central role in global beer production. This study investigates the price dynamics of its two key inputs—hops and malt—using monthly price data from 2015 to 2024 based on a unique dataset from a German hops cooperative. While contract farming is common for both raw materials, malt is traded via private firms, whereas hops are marketed almost exclusively through farmer-owned cooperatives. A vector error correction model (VECM) is estimated, incorporating structural break dummies identified through Bai–Perron tests, followed by forecast error variance decomposition (FEVD) and impulse response function (IRF) analysis. The results show that hop prices are largely self-driven and adjust more quickly to deviations from equilibrium than malt or beer prices. While malt and beer exhibit stronger interdependencies, the hop sector displays greater price stability. The findings further reveal that the malting sector responded significantly to the recent energy price crisis in Germany, whereas the hop sector did not.
The chicken gut microbiome in conventional and alternative production systems
(2025) Cheng, Yu-Chieh; Krieger, Margret; Korves, Anna-Maria; Camarinha‑Silva, Amélia
The poultry gut microbiome plays a key role in nutrient digestion, immune function, and overall health. Differences among various farming systems, including conventional, antibiotic-free, free-range, and organic systems, influence microbial composition and function through variations in diet, genetic selection, environmental exposure, and antibiotic use. Conventional systems typically rely on formulated diets and controlled housing conditions, often with routine antimicrobial use. In contrast, organic systems emphasize natural feed ingredients, including roughage, outdoor access, and strict limitations on the use of antibiotics. These divergent practices shape the gut microbiota differently, with organic systems generally associated with greater exposure to environmental microbes and, consequently, greater microbial diversity. However, the implications of this increased diversity for poultry health and performance are complex, as organic systems may also carry a higher risk of pathogen exposure. This review summarizes current findings on the chicken gut microbiome across conventional and alternative production systems (antibiotic-free, free-range, and organic), focusing on microbial diversity, functional potential, and disease resilience. The need for standardized methodologies and consistent nomenclature in microbiome research is also discussed to improve comparability across studies. Understanding how production systems influence the gut microbiota is essential for improving poultry health and productivity while addressing challenges related to antimicrobial resistance and sustainable farming practices.
Using landscape genomics to infer genomic regions involved in environmental adaptation of soybean genebank accessions
(2025) Haupt, Max; Schmid, Karl
Background: Understanding how crops adapt to specific environmental conditions is becoming increasingly important in the face of accelerating climate change, but the genetics of local adaptation remains little understood for many crops. Landscape genomics can reveal patterns of genetic variation that indicate adaptive diversification during crop evolution and dispersal. Here, we examine genetic differentiation and association signatures with environmental gradients in soybean ( Glycine max ) germplasm groups from China that were inferred from the USDA Soybean Germplasm Collection ( N = 17, 019 accessions) based on population structure and passport information. Results: We recover genes previously known to be involved in soybean environmental adaptation and report numerous new candidate genes in adaptation signatures implicated by genomic resources such as the genome annotation and gene expression datasets to function in flowering regulation, photoperiodism and stress reaction cascades. Linkage disequilibrium network analysis suggested functional relationships between genomic regions with signatures of genetic differentiation, consistent with a polygenic nature of environmental adaptation. We tested whether haplotypes associated with environmental adaptation in China were present in 843 North American and 160 European soybean cultivars and found that haplotypes in major genes for early maturity have been selected during breeding, but also that a large number of haplotypes exhibiting putative adaptive variation for cold regions at high latitudes are underrepresented in modern cultivars. Conclusions: Our results demonstrate the value of landscape genomics analysis of genebank accessions studying crop environmental adaptation and to inform future research and breeding efforts for improved adaptation of soybean and other crops to future climates.
Can market fragmentation explain the limited success of political attempts to promote grain legume cultivation in Germany?
(2025) Mittag, Franziska; Hess, Sebastian
Grain legumes, such as field peas, field beans, sweet lupins and soybeans, are known to increase biodiversity within the appropriate crop rotation and are an important source of import-substituting feed protein. National and regional policy schemes have long tried to support the cultivation of grain legumes. Although many regions in Germany offer favourable conditions for grain legumes, previous subsidy schemes have failed to increase the area under cultivation and farmers report a lack of market incentives. Indeed, the available price data exhibit a substantial share of non-random missing values in weeks when grain legumes were not traded. We analyse these non-price periods using cointegration tests and single-hurdle Tobit models. The results indicate that regional price formation for grain legumes in German regions depends not only on a minimum quantity of the respective legume crop in supply but also on a favourable supra-regional soybean price: Regional grain legume markets are not integrated and show evidence of a fragmented market structure. This may explain why local grain legume value chains have failed to emerge in Germany, despite decades of policy attempts to support these crops.
Systematic selection of best performing mathematical models for in vitro gas production using machine learning across diverse feeds
(2025) Ahmadi, Hamed; Titze, Natascha; Wild, Katharina; Rodehutscord, Markus
In vitro gas production (GP) is commonly used to evaluate ruminant feed, yet its accurate interpretation requires robust mathematical modeling. This study systematically explores a wide array of nonlinear models to explain GP dynamics across various feed types, addressing the question: how can efficient and versatile models that accurately represent GP profiles be identified? We hypothesized that distinct feed types exhibit unique GP characteristics, effectively captured by specific models, and that statistical and machine learning methodologies can streamline model selection. Utilizing a comprehensive dataset derived from 849 unique GP profiles across concentrate feed categories—including cereal and leguminous grains and processed protein feeds—21 candidate models were rigorously evaluated based on their goodness-of-fit metrics, with a particular emphasis on Bayesian Information Criterion (BIC) for model selection. A group of three models—namely Burr XII, Inverse paralogistic, and Log-logistic—consistently emerged as top performers, demonstrating high generalizability and predictive power across feed types. Notably, our analysis indicated that model type significantly influenced GP predictions, surpassing the impact of feed type characteristics. This research establishes a decision-making framework for model selection and sets the stage for further investigations linking in vitro GP parameters to in vivo digestibility, ultimately enhancing ruminant nutrition strategies.
Effects of Prosopis juliflora pods on sheep performance and carcass traits, and their methane mitigation potential as assessed in vitro
(2025) Tadesse, Assefa; Titze, Natascha; Rodehutscord, Markus; Melesse, Aberra
This study aimed to assess the effect of partial substitution of concentrate mix (CM) with Prosopis juliflora pod (PJP) on growth performance and carcass traits in sheep, and its potential to reduce methane (CH4) production in vitro. A total of 25 yearling rams with an initial body weight of 15.8±1.53 kg was randomly assigned to five treatment diets. The diets were a control diet (JP0) and JP0 replaced with PJP at the level of 5% (JP5), 10% (JP10), 15% (JP15), and 20% (JP20). Data were collected on feed intake, body weight, and carcass characteristics. Methane production was determined from 24 h in vitro gas production. The substitution of CM with PJP did not affect feed intake, body weight, weight gain, feed efficiency, and carcass traits (p>0.05). The in vitro CH4 production was reduced (p<0.001) in diets containing increased levels of PJP. The metabolizable energy for JP10, JP15 and JP20 diets was 11.9, 11.6 and 11.5 MJ/kg DM, respectively (p>0.05). In conclusion, replacing CM with PJP did not negatively affect growth performance and carcass characteristics and could be used as potential supplement to mitigate methane emissions. Further in vivo studies involving respiration chambers are recommended to investigate the CH4 reduction potential of PJP.
Iron partitioning and photosynthetic performance in Cannabis sativa L. reveal limitations of nanoscale zero-valent iron as a fertilizer
(2025) Büser, Christian; Hartung, Jens; Deurin, Lukas; Graeff-Hönninger, Simone
Iron (Fe) is the fourth most abundant element in the Earth’s crust but remains the third most limiting nutrient for crop productivity due to its low solubility in most soils. The emergence of nanotechnology has introduced nanoscale zero-valent iron (nZVI) as a potential Fe fertilizer with high surface reactivity and improved bioavailability. However, its comparative efficacy relative to conventional chelated Fe sources remains poorly understood. This study investigated Fe partitioning, photosynthetic efficiency, biomass accumulation, and cannabinoid synthesis in Cannabis sativa L. grown hydroponically under Fe-EDTA, nZVI, or Fe-deficient (-Fe) treatments. Total Fe concentrations were markedly reduced in -Fe plants compared with both Fe-EDTA and nZVI treatments. Despite similar root Fe contents between Fe-EDTA and nZVI, only Fe-EDTA facilitated efficient translocation to shoots, while nZVI-derived Fe predominantly accumulated in roots. Consequently, nZVI-treated plants exhibited intermediate photosynthetic performance and water-use efficiency—lower than Fe-EDTA but significantly higher than -Fe. Although Fe translocation differed substantially, inflorescence biomass and cannabinoid yield were comparable between nZVI and Fe-EDTA treatments, both exceeding those of -Fe plants. These results suggest that yield reductions under Fe deficiency arise not solely from Fe scarcity but also from the metabolic costs of Strategy I Fe acquisition, which are partially circumvented by root Fe availability from nZVI. Overall, Fe-EDTA demonstrated superior nutrient use efficiency, whereas nZVI partially alleviated Fe deficiency and revealed distinctive interactions between nanomaterials and plant Fe physiology. This study advances understanding of nZVI as an alternative Fe source in C. sativa and provides new insights into nanoparticle–plant nutrient dynamics.
Poultry perfection : comparison of computer vision models to detect and classify poultry products in a production setting
(2025) Einsiedel, Daniel; Vita, Marco; Jox, Dana; Dunnewind, Bertus; Meulendijks, Johan; Krupitzer, Christian
This study explores the use of computer vision, specifically object detection, for quality control in ready-to-eat meat products. We focused on a single process step, labeling products as “good” or “imperfect”. An “imperfect product” constitutes a product that deviates from the norm regarding shape, size, or color (having a hole, missing edges, dark particles, etc.). Imperfect does not mean the product is inedible or a risk to food safety, but it affects the overall product quality. Various object detectors, such as YOLO, including YOLO12, were compared using the mAP50-95 metric. Most models achieved mAP scores over 0.9, with YOLO12 reaching a peak score of 0.9359. The precision and recall curves indicated that the model learned the “imperfect product” class better, most likely due to its higher representation. This underscores the importance of a balanced dataset, which is challenging to achieve in real-world settings. The confusion matrix revealed false positives, suggesting that increasing dataset volume or hyperparameter tuning could help. However, increasing the dataset volume is usually the more difficult path since data acquisition and especially labeling are by far the most time-consuming steps of the whole process. Overall, current models can be applied to quality control tasks with some margin of error. Our experiments show that high-quality, consistently labeled datasets are potentially more important than the choice of the model for achieving good results. The applied hyperparameter tuning on the YOLO12 model did not outperform the default model in this case. Future work could involve training models on a multi-class dataset with hyperparameter optimization. A multi-class dataset could contain more specific classes than just “good” and “imperfect,” making trained models capable of actually predicting specific quality deviations.
The role of biodiversity in consumer research on food : a review and research agenda
(2025) Schneider, Deborah; Osieja, Sanja; Herbes, Carsten; Weinrich, Ramona
The move towards sustainable consumption has drawn significant attention from scholars and practitioners alike. Biodiversity is an integral part of environmental sustainability and food security, but it has received little attention in consumer research. Findings are fragmented into how biodiversity as a product attribute affects consumer food choices. Thus, we conducted a systematic literature review on consumer research on biodiversity in the context of food choice. We reviewed 45 papers published between 2014 and March 2025. Our analysis was guided by the TCCM (Theories, Contexts, Characteristics, and Methodology) framework for developing a systematic literature review. We examined how biodiversity was conceptualized and defined within the studies and towards study participants. Our findings show that biodiversity as a product attribute generally has positive effects on consumer attitude, willingness to pay, and product choice. However, knowledge about biodiversity and the inherent trade-offs in product choices remains limited, so consumers cannot realistically factor biodiversity into their decision-making. We find that research has taken a narrow view of biodiversity, qualitative studies are rare, and few efforts have been made to build theoretical perspectives from empirical data. We conclude with a proposed agenda for future research and a tentative definition of biodiversity for social science.
Structure formation in fruit preparations by fruit fermentates produced with exopolysaccharide-forming lactic acid bacteria
(2025) Festini, Silvan; Zipori, Dor; Wallisch, Marc; Weiss, Agnes; Neidhart, Sybille; Schmidt, Herbert; Jekle, Mario
Fruit preparations are intermediate food products that are primarily used in the dairy industry for the production of fruit yogurt or frozen desserts. Typically, they are stabilized by added hydrocolloids like pectins. The objective of this study was to investigate the potential replacement of conventional stabilizers by structure-forming fermentates produced by exopolysaccharides (EPS)-forming lactic acid bacteria (LAB). Peach puree was selected as fermentation matrix. Prior to 72 h of incubation, it was inoculated with either the heterofermentative LAB strain Levilactobacillus brevis TMW 1.2112 or the homofermentative LAB strain Pediococcus parvulus strain LTH 1110, both being known to produce EPS in form of β-D-glucan. The lyophilized fermentates were applied as stabilizers to produce strawberry fruit preparations. Flow curves, viscoelastic behaviour and shear stability were measured to investigate the effect of fermentate incorporation on the rheological properties of the products. A fermentatively induced effect was observed in terms of a 1.3-fold increase in viscosity of strawberry model fruit preparations with 10 % fermentate of Lv. brevis TMW 1.2112 compared to the addition of the same dose of fermentate blank. Further, increasing the fermentate blank dose from 10 % to 15 % resulted in a 2.4-fold viscosity increase of the model fruit preparations. High shear stability was found in all model strawberry fruit preparations. However, fermentation had no clear benefit in terms of viscoelastic behaviour and shear stability of the fruit preparations. Although the fermentatively induced thickening potential was limited, production of viscosity-increasing peach fermentate with minor changes in the sugar and amino acid profiles of the fruit proved to be feasible.
Expression of fibroblast growth factor 23 (FGF23) and αKlotho in two commercial laying hen strains fed with and without dietary mineral P supplements before and after the onset of the laying phase
(2025) Meier, Leonie; Wallauch, Nadine; Feger, Martina; Oster, Michael; Sommerfeld, Vera; Schmucker, Sonja; Wimmers, Klaus; Huber, Korinna; Stefanski, Volker; Rodehutscord, Markus; Föller, Michael
Maintenance of phosphate homeostasis is particularly critical in laying hens for bone formation and calcium mobilization. The supplementation of their feed with mineral phosphate is common although recent research questions the usual levels of supplementation. Phosphate homeostasis is classically regulated by active vitamin D (calcitriol) and parathyroid hormone, whereas fibroblast growth factor 23 (FGF23) and its co-receptor αKlotho are novel factors. FGF23 has emerged as an important disease biomarker and αKlotho as an anti-aging factor in mammals, however, little is known about their role in poultry. Here, we studied FGF23 and αKlotho expression in two commercial laying hen strains under conditions of dietary mineral phosphorus renunciation and sufficient phosphorus supply. Fifteen- and 20-week-old Lohmann Brown-Classic (LB) or LSL-Classic (LSL) hens were fed a standard maize-soybean-based diet containing 0 or 1 g/kg additional mineral phosphorus for 4 weeks. The animals were sacrificed, and gene expression studied in different organs by quantitative real-time PCR and protein expression by western blotting. Statistical correlation with further parameters of mineral metabolism was analyzed by Pearson’s correlation coefficient or Spearman’s Rho. As a result, FGF23 bone expression was significantly lower and hepatic FGF23 expression higher in 24-week-old than in 19-week-old hens. Bone, hepatic, and renal αKlotho expression was significantly higher in older than younger animals. Compared to LB hens, LSL hens exhibited higher hepatic αKlotho irrespective of diet and age. Dietary phosphorus content did not significantly affect FGF23 and αKlotho expression. Bone FGF23 expression was positively and hepatic FGF23 negatively associated with plasma phosphate concentration whereas bone FGF23 expression was negatively and hepatic FGF23 positively associated with plasma calcitriol concentration. To conclude, we uncovered a strong impact of age and strain on FGF23 and αKlotho expression in two high performance laying hen strains, effects possibly associated with initiation of the egg-laying phase. Moreover, the regulation of hepatic FGF23 expression differed from the regulation of bone FGF23 expression. Further studies are needed to elucidate the physiological relevance.
From import to establishment? Experimental evidence for seasonal outdoor survival of two Rhipicephalus species in Germany
(2025) Fachet-Lehmann, Katrin; Lindau, Alexander; Mackenstedt, Ute
The brown dog tick (Rhipicephalus sanguineus s.l.), though not endemic in Germany, is regularly introduced via travelers with dogs and imported rescue dogs. Due to its relevance in veterinary and human medicine, its potential to establish in Germany’s climate is of interest. Although previous studies confirm indoor survival and reproduction of R. sanguineus s.s. in Germany, climate change and milder winters may also allow outdoor survival. This study assessed the survival of R. sanguineus s.s. and R. innaei from February 2023 to May 2024 using laboratory-bred ticks placed at indoor and outdoor sites. Tick survival (adults, nymphs, larvae) was monitored weekly, along with temperature and humidity. Reproductive success was evaluated via oviposition and larval hatching. R. sanguineus s.s. adults survived up to 44 weeks, nymphs up to 20 weeks, and larvae up to 5 weeks. R. innaei showed shorter survival (37, 10, and 4 weeks, respectively). Successful oviposition and larval hatching occurred outdoors between May´23 and September´23 for both species. However, winter survival was not observed; all ticks died following sub-zero temperatures in December´23. Despite the inability to overwinter outdoors, both species can survive for extended periods in spring and summer and may enter homes via dogs, where conditions favor year-round survival. Their ability to transition indoors via dogs, where conditions favor year-round survival, suggests a potential for establishment in Germany through combined indoor and seasonal outdoor persistence.
Bioaccessibility and anti-inflammatory activity in Caco-2 cells of phytochemicals from industrial by-products of coffee (Coffea arabica L.)
(2025) Jiménez-Gutiérrez, Milena; Zielinski, Christian; Esquivel, Patricia; Frank, Jan; Irías-Mata, Andrea; Jiménez-Aspee, Felipe
Coffee by-products are rich in nutrients and bioactive compounds in free soluble form and bound to cell wall components. These compounds undergo chemical changes during gastrointestinal digestion, affecting their bioaccessibility and bioactivity. This study is the first to investigate coffee by-products from industrial wet processing to evaluate the impact of simulated gastrointestinal digestion on their phytochemical composition and subsequent anti-inflammatory activity in Caco-2 cells. Digestion significantly reduced the stability and solubility of main compounds; however, digested bioaccessible by-products still exhibited anti-inflammatory properties, reducing IL-6, IL-8, and TNF-α levels. Correlation analysis identified rutin, quercetin-3-glycoside, caffeine and 5-caffeoylquinic acid as strongly linked to cytokine suppression, suggesting key roles and possible synergies. These results highlight the potential of coffee by-products as functional ingredients targeting intestinal inflammation. Future work should confirm in vivo efficacy, optimize extraction at scale, and address regulatory requirements to support industrial application and promote circular economy benefits.
Sulfate enhances metabolic acclimation under drought stress - a comparative study of grapevine and maize
(2025) Lehr, Patrick Pascal; Erban, Alexander; Kopka, Joachim; Zörb, Christian
The importance of sulfate fertilization in plant production has becoming increasingly relevant due to the decline in atmospheric sulfur inputs. Moreover, high-intensity cropping systems are increasingly facing drought scenarios. The role of stomata is crucial during drought stress and is linked to sulfate metabolism. This study investigates the impact of sulfate application on the drought response of grapevine and maize guard cells. Both plant species may serve as crop model for analysing underlying physiological processes in a low fertilizer demanding crop such as grapevine and a high fertilizer demanding crop such as maize. Increased sulfate concentration in leaves was triggered by drought in maize and grapevine, but in grapevine only when additional sulfate was applied. Additional sulfate application improved sulfur availability under drought conditions, which enhanced drought stress response in grapevine and maize. This was characterized by enhanced metabolic acclimation under drought conditions. The effect of sulfate on the drought stress response was markedly diminished in guard cells, indicating enhanced metabolic stability of guard cells against external influences. These results underscore the significance of adequate sulfate supply to crops for optimal drought stress response and suggest that sulfate fertilisation may serve as a potential option to enhance drought acclimation
Historic insights and future potential in wheat elaborated using a diverse cultivars collection and extended phenotyping
(2025) El Hassouni, Khaoula; Afzal, Muhammad; Boeven, Philipp H. G.; Dornte, Jost; Koch, Michael; Pfeiffer, Nina; Pfleger, Franz; Rapp, Matthias; Schacht, Johannes; Spiller, Monika; Sielaff, Malte; Tenzer, Stefan; Thorwarth, Patrick; Longin, C. Friedrich H.
Wheat is one of the most important staple crops worldwide. Wheat breeding mainly focused on improving agronomy and techno-functionality for bread or pasta production, but nutrient content is becoming more important to fight malnutrition. We therefore investigated 282 bread wheat cultivars from seven decades of wheat breeding in Central Europe on 63 different traits related to agronomy, quality and nutrients in multiple field environments. Our results showed that wheat breeding has tremendously increased grain yield, resistance against diseases and lodging as well as baking quality across last decades. By contrast, mineral content slightly decreased without selection on it, probably due to its negative correlation with grain yield. The significant genetic variances determined for almost all traits show the potential for further improvement but significant negative correlations among grain yield and baking quality as well as grain yield and mineral content complicate their combined improvement. Thus, compromises in improvement of these traits are necessary to feed a growing global population.
Effect of liquefaction temperature and enzymatic treatment on bioethanol production from mixed waste baked products
(2025) Almuhammad, Mervat; Kölling, Ralf; Einfalt, Daniel
This study investigates the effect of different liquefaction temperatures (50–70 °C) and four commercial enzyme formulations on glucose release and subsequent ethanol yield, using mixed waste baked products as a substrate. Among the enzymes tested, Amylase GA 500 proved to be superior in the hydrolysis of starch at lower temperatures (50 °C and 55°C). At higher liquefaction temperatures (65 °C and 70°C) all four enzyme preparations showed comparable activity. The highest glucose concentration (205.7 g/L) and the highest ethanol yield (92 g/L) were achieved with Amylase GA 500 at 65 °C. Its superior performance is attributed to the synergistic activity of α-amylase and glucoamylase, which facilitates efficient starch hydrolysis. Crucially, we discovered that the liquefaction temperature profoundly affects fermentation speed independently of the initial glucose concentration or the enzyme preparation used for starch hydrolysis. This novel mechanistic insight suggests that higher temperature treatment either makes an additional factor crucial for yeast fermentation available or depletes/destroys an inhibitor present in the complex waste bakery product matrix. These findings highlight the critical role of temperature and enzyme formulation in optimizing bioethanol production from bakery waste, supporting the development of more sustainable and efficient waste-to-biofuel processes.
Towards sustainable biointelligent food design: structuring potential of plant-based materials exemplified using apricot seed oil oleogels and bigels through 3D food printing
(2025) Reinmuth, Evelyn; Fahmy, Ahmed Raouf; Ribette, Olivia; Jekle, Mario
Background/Introduction: Biointelligence in the approach of food additive manufacturing represents a significant advancement, enabling the reverse engineering and design of foods. Legislation restricting trans-fats has accelerated research into alternatives, but ingredients like saturated and trans fats play key roles in food quality and functionality. Oleogels are a promising replacement. Food additive manufacturing introduces a biointelligent approach, combining biological and technical components with information technology to optimize food design. This study investigates 3D printing of oleogel and bigel systems using apricot seed oil, aiming to assess their significance, applicability, and printability as sustainable alternatives to trans fats for innovative, resource-efficient food production. Methods: Apricot seed oil, rich in antioxidants and polyunsaturated fatty acids, was processed into plant-based oleogels and bigels. The material systems were incorporated into 3D printed food structures. Material characterization and techno-functional analysis were conducted to evaluate the suitability of apricot seed oil for structuring 3D printed foods and controlling food texture. Results: Adjusting the type and concentration of oil-gelator mixtures enabled tailored texture and lipid distribution to fit consumer preferences. Sustainability impacts were assessed at intermediate processing steps, demonstrating the value of holistic evaluations beyond technical factors. Discussion: Biointelligent 3D printing offers a platform to optimize sensory and sustainability qualities in food design. The integration of apricot seed oil into novel food matrices enables versatile nutritional product development, supporting researchers and industry stakeholders in advancing consumer-centric, sustainable production and consumption practices.

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