Mating-type analysis in Diaporthe isolates from soybean in central Europe

dc.contributor.authorHosseini, Behnoush
dc.contributor.authorKäfer, Lena Sophia
dc.contributor.authorLink, Tobias Immanuel
dc.contributor.editorCai, Lei
dc.date.accessioned2025-06-04T13:05:08Z
dc.date.available2025-06-04T13:05:08Z
dc.date.issued2025
dc.date.updated2025-05-13T11:20:58Z
dc.description.abstractSpecies of the genus Diaporthe have a mating-type system with the two mating types MAT1-1 and MAT1-2, like other ascomycetes. They can either be heterothallic, which means that any isolate only possesses one of the two mating types and needs a mating partner for sexual reproduction, or homothallic, which means that they possess both mating types and are self-fertile. For several Diaporthe species, no sexual reproduction has been observed so far. Using PCR with primers specific to the defining genes MAT1-1-1 and MAT1-2-1 , we determined the mating types of 33 isolates of Diaporthe caulivora , D. eres , D. longicolla , and D. novem from central Europe. In addition, we partially sequenced the mating-type genes of 25 isolates. We found that different D. longicolla isolates either possess MAT1-1-1 or MAT1-2-1, making the species heterothallic, which is in contrast to previous studies and the general assumption that D. longicolla only reproduces asexually. D. eres and D. novem were also found to be heterothallic. Using genomic sequence information and re-sequencing of DNA and RNA, we identified the MAT1-1-1 gene in D. caulivora and present here the full sequence of the mating-type locus of this homothallic species. Finally, we used sequence information from MAT1-1-1 and MAT1-2-1 , respectively, for improved phylogenetic resolution of our isolates.
dc.description.sponsorshipThis research was funded by the German Federal Ministry of Food and Agriculture, grant number 2815EPS082 (SoySound), to T.I.L. Publishing fees supported by Funding Programme Open Access Publishing of University of Hohenheim.
dc.description.sponsorshipGerman Federal Ministry of Food and Agriculture
dc.identifier.urihttps://doi.org/10.3390/jof11040251
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/17701
dc.language.isoeng
dc.rights.licensecc_by
dc.subjectMating-type genes
dc.subjectMating-type locus
dc.subjectDiaporthe longicolla
dc.subjectDiaporthe caulivora
dc.subjectPhylogeny
dc.subject.ddc570
dc.titleMating-type analysis in Diaporthe isolates from soybean in central Europeen
dc.type.diniArticle
dcterms.bibliographicCitationJournal of Fungi, 11 (2025), 4, 251. https://doi.org/10.3390/jof11040251. ISSN: 2309-608X
dcterms.bibliographicCitation.articlenumber251
dcterms.bibliographicCitation.issn2309-608X
dcterms.bibliographicCitation.issue4
dcterms.bibliographicCitation.journaltitleJournal of Fungi
dcterms.bibliographicCitation.originalpublishernameMDPI
dcterms.bibliographicCitation.originalpublisherplaceBasel
dcterms.bibliographicCitation.volume11
local.export.bibtex@article{Hosseini2025, doi = {10.3390/jof11040251}, author = {Hosseini, Behnoush and Käfer, Lena Sophia and Link, Tobias Immanuel et al.}, title = {Mating-Type Analysis in Diaporthe Isolates from Soybean in Central Europe}, journal = {Journal of Fungi}, year = {2025}, volume = {11}, number = {4}, }
local.export.bibtexAuthorHosseini, Behnoush and Käfer, Lena Sophia and Link, Tobias Immanuel et al.
local.export.bibtexKeyHosseini2025
local.export.bibtexType@article
local.title.fullMating-Type Analysis in Diaporthe Isolates from Soybean in Central Europe

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